MC1R

Tunde73, 13-8-2012 15:31:

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I reacted on this your phrase:
"... 1 ER (Fayoumi) amino acid with 1 eWh (wheaten) amino acid // 1 ER (Fayoumi) amino acid with 1 eb (brown) amino acid"

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Danny_57, 13-8-2012 16:21:

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1. About the mutation on position 213 probably having no/little effect on phenotype was given me first-handed by a person who was co-worker on the elucidation of MC1R.

2. That was exactely why my question was made here to professional(s) (see the subtitle) to give an answer that could help us simple breeders.

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Tunde73, 13-8-2012 17:29:

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But this: "Leu133Gln" isn't an aminoacid...... Aminoacids are leucine, glutamine and so on.
I did not understand your question.

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Danny_57, 13/08/2012 18.27:

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When the amiono acid "leucine" who is at position 133 on the MC1R mutated to "glutamine" it is written shortened as "Leu133Gln", even more shorten is to write it as "K133Q" since the amino acid abbreviations for leucine is "K" and for glutamine is "Q"

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Tunde73, 13-8-2012 18:30:

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Now you wrote it correct but before wasn't correct!

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Tunde73, 13-8-2012 17:38:

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This: "Met71Met, Glu92Glu, Leu133Leu, Thr143Thr, His215His" does mean nothing. If they are no mutations, Thr143Thr (and so on) (nothing changed in position 143) is not correct to write, threonine has remained threonine.

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Tunde73, 13-8-2012 15:31:

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(I think they took as original also Cys in position 213, otherwase, they had to indicate in all mutations also the mutation Arg/Cys.)

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Danny_57, 13/08/2012 18.42:

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Aahh ... ok than, I am glad it is as you want now :-) even I don't see a difference with post #1.

Could you now also please answer the question in post #1 ?

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Danny_57, 13/08/2012 18.42:

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Aahh ... ok than, I am glad it is as you want now :-) even I don't see a difference with post #1.

Could you now also please answer the question in post #1 ?

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Tunde73, 13-8-2012 21:27:

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Publications not write "aminoacid: His214Pro"........
You must write a sequence, alone this mutations say nothing. But I immagine you are meaning (refering on the E locus) a chicken with genotype ER/ewh and an other with genotype ER/e+. The question is not right but I think I understood you. You know which phenotype have an ER/e+ chickens or an ER/ewh chickens, better of me, so if was this your question, I not understand why you did it.

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Tunde73, 13-8-2012 21:46:

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Danny, if you are asking seriously what phenotyoe is this and you are refering on 1 chicken with 1 allele with 2 mutations and with the other allele with other 2 mutations, this phenotype not exist because the mutation Leu133Gln and Thr143Ala together, on the E locus not exis, for now it's no allele like this as it's no for now allele with the other 2 mutations. Or maybe exist but we know nothing about, for now. (Or an other possibility, I not readed about this new mutation.)

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Tunde73, 13-8-2012 22:10:

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Nobody sayd that it is strange more mutations together on the same locus.
Yes, in this way (His215 to Pro) everybody understand you...

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Danny_57, 13/08/2012 19.36:

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Here you can see what if is a difference or not (under "line" => RJF)

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Tunde73, 13-8-2012 22:28:

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Sapevo già la differenza tra le 2 sequenze, ciò che stavo dicendo è che per forza hanno preso come allele originale quello con la cisteina, in posizione 213 perchè se avessero preso come originale la sequenza con arginina, nella stessa posizione, per tutte le mutazioni (E, ER, ewh and so on) avrebbero dovuto indicare in più la mutazione avvenuta in questa posizione, visto che tutti gli altri alleli (in posizione 213) hanno una sequenza di basi che codifica per la cisteina...

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Tunde73, 13-8-2012 22:42:

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Cosa sono questi punti interrogativi?

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